Description

Size

50µg

Catalog no.:

PKSH031803-50µg

Just click and buy

Quick buy process

We invite you to purchase this product.                                     Our professional team will fulfill your order as soon as possible.                                     Our experienced employees will take care of your order very quickly.

All details

Background

Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed exclusively in the nervous system. The subgroup consists of six members: Contactin-1, Contactin-2(TAG-1), Contactin-3(BIG-1), BIG-2, Contactin-5(NB-2) and NB-3. Since their identification in the late 1980s, Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast, the function of the other four members of this subgroup remained unknown until recently. Contactin-3, also known as CNTN3 ( BIG-1 in rat and PANG in mouse ), is a GPI-linked glycoprotein that is expressed on cerebellar Purkinje cells, amygdaloid and thalamic neurons and olfactory granule cells. In the brain, Contactin-3 is expressed in frontal lobe, occipital lobe, cerebellum and amygdala. Contactin-3 contains 4 fibronectin type-III domains and 6 Ig-like C2-type (immunoglobulin-like) domains. Human Contactin-3 shares 92% aa identity with mouse Contactin-3.The exact function of Contactin-3 is unclear. Contactin-3 may mediate cell-cell interaction and may promote neurite outgrowth.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Activity

Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 10E4 cells/well are added to CNTN3 coated plates (0.8 μg/ml and 100 μl/well), approximately 30%-50% will adhere specifically after 60 minutes at 37℃.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability and Storage

Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Formulation

Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5

Endotoxin

<1.0 EU per µg as determined by the LAL method

Purity

>90 % as determined by SDS-PAGE

Source

Recombinants or rec. proteins

Sequence

Met 1-Ser 1002, 708 Asp/Asn

Synonym

BIG-1;PANG;PCS

Expressed Host

HEK293 Cells

Group

recombinants

AP Mol Mass

160-170 kDa

Accession

NP_065923.1

Mol Mass

134.5 kDa

Fusion tag

C-Fc