Description

Size

96Test

Catalog no.:

E-CL-R0045-96T

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Test principle

This CLIA kit uses the Sandwich- CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PAPPA . Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PAPPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PAPPA , biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PAPPA . You can calculate the concentration of Rat PAPPA in the samples by comparing the RLU value of the samples to the standard curve.

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Target Synonym

PAPP-A, PAPA, DIPLA1, IGFBP-4ase, PAPPA1, ASBABP2, Pappalysin 1

Product Name

Rat PAPPA (Pregnancy Associated Plasma Protein A) CLIA Kit

Tested Sample Types

Serum, Plasma, Cell supernatant

Latin name

Rattus norvegicus

Detection Range

31.25~2000pg/mL

Detection Type

Colormetric

Sensitivity

18.75pg/mL

Type

Sandwich

Target Name

PAPPA

Target Species

Rat

Cleaverplas