Cleaverplas

Mycoplasma is a genus of bacteria that lack a cell wall around their cell membrane. Without a cell wall, they are unaffected by many common antibiotics such as penicillin or other beta-lactam antibiotics that target cell wall synthesis. They can be parasitic or saprotrophic. Several species are pathogenic in humans, including M. pneumoniae, which is an important cause of atypical pneumonia and other respiratory disorders, and M. genitalium, which is believed to be involved in pelvic inflammatory diseases. Mycoplasma species are the smallest bacterial cells yet discovered, can survive without oxygen, and come in various shapes. For example, M. genitalium is flask-shaped (about 300 x 600 nm), while M. pneumoniae is more elongated (about 100 x 1000 nm). Hundreds of mycoplasmas infect animals

The lyophilized Mycoplasma Arginine Deiminase recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted Mycoplasma Arginine Deiminase stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Mycoplasma Arginine Deiminase recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Arginine Deiminase (ADI) is a microbial enzyme from Mycoplasma produced in E.coli. It has high affinity to L-arginine and hydrolyzes L-arginine to citrulline and ammonia. Low concentrations of ADI have been shown to inhibit proliferation in certain cultured cells by arresting the cell cycle in G (1) and/or S phase. Higher concentrations of ADI lead to subsequent apoptosis. Recombinant Mycoplasma Arginine Deiminase is a 46.4 kDa protein consisting of 409 amino acids.

MSVFDSKFKG IHVYSEIGEL ESVLVHEPGR EIDYITPARL DELLFSAILE SHDARKEHKQ FVAELKANDI NVVELIDLVA ETYDLASQEA KDKLIEEFLE DSEPVLSEEH KVVVRNFLKA KKTSRELVEI MMAGITKTDL GIEADHELIV DPMPNLYFTR DPFASVGNGV TIHYMRYKVR QRETLFSRFV FSNHPKLINT PWYYDPSLKL SIEGGDVFIY NNDTLVVGVS ERTDLQTVTL LAKNIVANKE CEFKRIVAIN VPKWTNLMHL DTWLTMLDKD KFLYSPIAND VFKFWDYDLV NGGAEPQPVE NGLPLEGLLQ SIINKKPVLI PIAGEGASQM EIERETHFDG TNYLAIRPGV VIGYSRNEKT NAALEAAGIK VLPFHGNQLS LGMGNARCMS MPLSRKDVKW

Biological activity was measured by its ability to induce apoptosis in Jurkat cells using a concentration of 100-150 ng/mL. Endotoxin level is less than 0.1 ng per μg (1EU/μg).

arginine deiminase (ADI) (Arginine dihydrolase) (AD)

Greater than 97% by SDS-PAGE gel and HPLC analyses.

This product is for research use only.

Recombinants or rec. proteins

Mycoplasma crocodyli MP145

Mycoplasma crocodyli

YP_003560236.1

recombinants

Lyophilized

mycoplasma

MCRO_0627

294155852

8970640

E. coli

D5E643