Cleaverplas

Retinol binding protein 4, plasma, also known as RBP4, belongs to the lipocalin family and is the specific carrier for retinol(vitamin A alcohol) in the blood. It is protein that it is encoded by the RBP4 gene. RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. The mouse Rbp4 locus is closely linked and just proximal to the locus for phenobarbital-inducible cytochrome P450-2c(Cyp-2c) at the distal end of chromosome 19. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. The standard product used in this kit are recombinant mouse B7-1/CD80, D37—K245, consisting of dimer acids with two single straned. The standard used in this kit is recombinant protein, with E19-L201 aa sequence, the molecular weight is 22kda.

Sample the amount of 100ul/well of the standard solution at 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml mouse RBP4 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, serum, plasma(heparin , EDTA) or urine to each empty well. See “Sample dilutions Guideline” above for details. We recommend that each mouse RBP4 standard solution and each sample is measured in duplicate.

GENTAUR's mouse RBP4 ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for RBP4 was used as capure antibodies on the ELISA test plate. The internal standards (NSO, E19-L201) and your samples are measured with a biotin conjugated polyclonal from goat specific for RBP4 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse RBP4 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.

Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse RBP4 antibodies;1 ST0000-20;Lyophilized recombinant mouse RBP4 standard;20ng/tube×2 AR1107;Biotinylated anti- mouse RBP4 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Plasma retinol binding protein;Plasma retinol-binding protein;Plasma retinol-binding protein(1-176);PRBP;PRO2222;RBP;RBP4;RDCCAS;RET4_HUMAN;Retinol binding protein 4;Retinol binding protein 4 interstitial;Retinol binding protein 4 plasma

intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (ng/ml);1.34;4.6;10.12;1.65;4.34;9.86 ELISA standard deviations 0.06;0.348;0.617;0.102;0.304;0.76 CV(%);4.5;5.7;6.1;6.2;7;7.7

Concentration(pg/ml) + 0 + 312 + 625 + 1250 + 2500 + 5000 + 10000 + 20000 O.D. + 0.011 + 0.351 + 0.695 + 1.170 + 1.562 + 1.759 + 1.985 + 2.216

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

For quantitative measurement of mouse RBP4 in cell culture supernatant, serum, plasma heparin, EDTA and urine.

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.

the ELISA does not cross react with any other proteins except from closely related species

Natural and recombinant mouse RBP4

retinol binding protein 4, plasma

Retinol-binding protein 4

312pg/ml to 20000pg/ml

heparin or EDTA

NSO, E19-L201

Mus musculus

murine RBP4

< 10pg/ml

Q00724

15-20

19662