Cleaverplas

A copper-containing protein in Chlamydomonas reinhardtii is a plasma membrane ferroxidase, encoded by the FOX1 gene, involved in Fe assimilation. FOX1 is most highly related to the mammalian homologues hephaestin and ceruloplasmin; its occurrence and pattern of expression in Chlamydomonas indicate a role for copper in iron assimilation in a photosynthetic species.

Kropat et al. (2015). Copper economy in Chlamydomonas: Prioritized allocation and reallocation of copper to respiration vs. photosynthesis. Proc Natl Acad Sci U S A. 2015 Feb 2. pii: 201422492.LaFontaine et al. (2002) Copper-Dependent Iron Assimilation Pathway in the Model Photosynthetic Eukaryote Chlamydomonas reinhardtii. Eukaryotic Cell 1(5):736-757

This antibody has been used on Chlamydomonas cells expressing endogenous Fox1 and on HEK293 cells transiently expressing Fox1. Cells were resuspended in PBS plated on poly-lysine coated coverslips, fixed in 3.7% formaldehyde. Blocked in 3% BSA in PBS. 1:500 dilution of anti-FOX1 in 3% BSA. Secondary antibody was anti-rabbit Alexa fluor 488 1:300.

Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae. 

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

FOX1 is 10-20-fold induced in iron-deficiency and its occurrence therefore serves as a marker of iron nutrition state in Chlamydomonas. For detection image please refer to the article below.

AS07 260 | anti-H+ATPase, plasma membrane rabbit marker antibodyCollection of antibodies to Chlamydomonas proteinsAlgal protein extraction buffer Secondary antibodies

residues 394-646 from Chlamydomonas reinhardtii FOX1 A8IZT9 fused to TrxA

no confirmed exceptions from predicted reactivity known in the moment

For reconstitution add 200 µl of sterile water.

western blot (WB), immunofluorescence (IF)

1: 1000 - 1: 5000 with standard ECL (WB),

Chlamydomonas reinhardtii

Chlamydomonas reinhardtii

394646 , A8IZT9

Refer to NCBI

122 | 120 kDa

Lyophilized

Polyclonal

Polyclonal

Rabbit

Serum