Cleaverplas

Li et al. (2015). Three SAUR proteins SAUR76, SAUR77 and SAUR78 promote plant growth in Arabidopsis. Sci Rep. 2015 Jul 24;5:12477. doi: 10.1038/srep12477. Kolb et al. (2015). FYVE1 is essential for vacuole biogenesis and intracellular trafficking in Arabidopsis thaliana. Plant Physiol. 2015 Feb 19. pii: pp.114.253377. Hu et al. (2015). Re-examination of chlorophyllase function implies its involvement in defense against chewing herbivores. Plant Physiol. 2015 Jan 12. pii: pp.114.252023. Komatsu et al. (2014). Phototropin Encoded by a Single-Copy Gene Mediates Chloroplast Photorelocation Movements in the Liverwort Marchantia polymorpha L. 1. Plant Physiol. 2014 Sep;166(1):411-27. doi: 10.1104/pp.114.245100. Epub 2014 Aug 5. Berthier et al. (2014).Identification of a new sucrose transporter in rye-grass (LpSUT2): Effect of defoliation and putative fructose sensing. Plant Physiol & Biochem, vol: 84, Nov. 2014. (immunolocalization, leaf sheath) Migocka et al. (2014). Molecular and biochemical properties of two P 1B2 -ATPases, CsHMA3 and CsHMA4, from cucumber. Plant Cell Environ. 2014 Sep 11. doi: 10.1111/pce.12447. Shen et al. (2014). The fronds tonoplast quantitative proteomic analysis in arsenic hyperaccumulator Pteris vittata L. J Proteomics. 2014 Feb 4. pii: S1874-3919(14)00047-5. doi: 10.1016/j.jprot.2014.01.029. Zhang et al. (2014). Arabidopsis ABCG14 protein controls the acropetal translocation of root-synthesized cytokinins. Nat Commun. 2014 Feb 11;5:3274. doi: 10.1038/ncomms4274. Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.

KLH-conjugated synthetic peptide exposed to cytoplasm in H+ATPase model, derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (UniProt: P20649, TAIR:At2g18960) and ATPase 2 (UniProt: P19456 , TAIR:At4g30190),3 (UniProt: P20431, TAIR:At5g57350),4 (UniProt: Q9SU58, TAIR:At3g47950),6 (UniProt: Q9SH76, TAIR:At2g07560),7 (UniProt: Q9LY32, TAIR:At3g60330),8 (UniProt: Q9M2A0, TAIR:At3g42640),9 (UniProt: Q42556, TAIR:At1g80660), 11 (UniProt: Q9LV11, TAIR:At5g62670) of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3)

dicots including: Arabidopsis thaliana, Cucumis sativus, Cucurbita moschata, Glycine max (weak), Kandelia obovata, Lolium perenne, Lycopersicon esculentum, Marchantia polymorpha, Medicago truntata, Nicotiana tabacum, Noccaea caerulescens, Phalenopsis Sogo Yukidian cultivar V3, Ricinus communis, Spinacia oleracea, monocots inlcuding: Hordeum vulgare, Oryza sativa, Zea mays, trees: Picea abies, Populus tremula, Pteris vittata (fern), algae: Chlamydmonas reinhardtii

The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Do not store this antibody in 4°C.

Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae. 

dicots including: Mesembruanthemum crystallinum, Solanum tuberosum; monocots (including Avena sativa, Hordeum vulgare, conifers (Pinus thunbergii), mosses (Physocomitrella patens), algae (Dunaliella spp., Ostreococcus spp.), Saccharomyces cerevisiae

AS07 213 | anti-V-ATPase rabbit antibodyAS07 260PRE | H+ATPase | plasma membrane H+ATPase, pre-immune serumAS13 2671 | H+ATPase plasma membrane H+ATPase (chicken antibody) antibodies to membrane transport systemrecommended secondary antibody

for additional Western blot detection image please refer to the article below.VERY IMPORTANT: please, do not heat up your samples over 70°C as this might cause H+ATPase to precipitate and there will be no signal on your western blot.

1: 1000 - 1: 5000 with standard ECL OR 1: 10 000 with ECL-Advance, enhanced chemiluminescence (WB), 1: 600 - 1: 1000 (IF), 1: 100 (IL)

P20649 , P19456 , P20431 , Q9SU58 , Q9SH76 , Q9LY32 , Q9M2A0 , Q9M2A0 , Q42556 , Q9LV11 , Q9FNS3

At2g18960,At4g30190,At5g57350,At3g47950,At2g07560, At3g60330,At3g42640,At1g80660,At5g62670,

western blot (WB), immunofluorescence (IF), immunolocalization (IL)

For reconstitution add 50 µl of sterile water to each tube

cellular [compartment marker] for plasma membrane

95 kDa (Arabidopsis thaliana)

Aspergillus niger

Lyophilized

Polyclonal

Polyclonal

Rabbit

Serum