Cleaverplas

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Antiboy will weakly react with PIP2;1 and theoretically with PIP2;3, however this protein is very scarcely expressed in plant tissues. The mRNA content is 1 % of that for PIP2;2, therefore reactivity to PIP2;3 was neglegible in our experiments.Manufactured by Operon Biotechnology.

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Protocol for plant plasma membrane isolation can be found here.

Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae. 

collection of antibodies to plasma membrane proteins AS09 488 | anti-PIP2;1 | aquaporin PIP2;1AS09 489 | anti-PIP1;1, PIP1;2, PIP1;3 | aquaporinsAS09 491 | anti-PIP2;1,PIP2;2,PIP2;3 |plasma membrane intrinistic protein 2-1,2-2, 2-3Plant protein extraction bufferSecondary antibodies

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

KLH-conjugated synthetic peptide derived from Raphanus sativus PAQ2b Q9MB99

no confirmed exceptions from predicted reactivity known in the moment

Arabidopsis thaliana, Fragaria ananassa, Raphanus sativus

See included datasheet or contact our support service

1: 8000 (ELISA), 1: 2000 with standard ECL (WB)

PIP2;2 is a plasma membrane aquaporin.

ELISA (ELISA), western blot (WB)

30 | 28 kDa (Raphanus sativus)

dicots including: Brassica sp.

to be added when available

Polyclonal

Polyclonal

AT2G37170

Inquire

Rabbit

Q9MB99

Serum