Description
Size
100 µl
Catalog no.:
AS12 2110
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All details
Possible reactivity
Arabidopsis thaliana, Brassica oleracea, Cicer arietinum, Coffea arabica, Cucumis sativus, Fragaria chiloensis, Glycine max, Medicago trunculata, Mimosa pudica, Nicotiana tabacum, Olea europaea, Phaseolus vulgaris, Pisum sativum, Pyrus communis, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, Triticum urartu, Vitis vinifera,monocots: Hordeum vulgare, Oryza sativa, Triticum aestivum, trees: Picea mariana, Populus trichocarpa
Immunogen
KLH-conjugated synthetic peptide derived from Zea mays PIP2-7 C-terminal, Q9ATM4, conserved also in Zea mays PIP2-1, UniProt: Q84RL7, PIP2-2, UniProt: Q9ATM8, PIP2-3 (80 % conservation) UniProt: Q9ATM7, PIP2-4 (80 % conservation) UniProt: Q9ATM6, PIP2-5 (70 % conservation) UniProt: Q9XF58, PIP2-6 (50 % conservation) UniProt: Q9ATM5
Description
Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae.
Scientific context
Plasma membrane aquaporin, PIP2;7 is water channel protein required for water transport across cell membrane. Alternative names: plasma membrane intrinsic protein 2-7, AtPIP2;7, plasma membrane intrinsic protein 3, salt stress-induced major intrinsic protein, PIP3a
Storage condition
store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Connected products
AS09 488 | PIP2;1 | aquaporin PIP2;1AS09 490 | PIP2;2 | plasma membrane aquaporin 2bAS09 491 | PIP2;1,PIP2;2,PIP2;3 |plasma membrane intrinistic protein 2-1,2-2, 2-3Plant protein extraction bufferSecondary antibodies
Notes
Protocol for isolation of plant plasma membrane proteins can be found here.This antibody has a potential to work in immunolocalization studies, as it is recognizing C-terminal part of the sequence.
No reactivity
no confirmed exceptions from predicted reactivity known in the moment
Verified reactivity
Lactuca sativa, Pisum sativum, Solanum lycopersicum, Zea mays
References
to be added when available, antibody released in May 2012.
Supplementary information
detection pattern consists of di and monomer of PIP2-7
How to reconstitute
For reconstitution add 100 µl of sterile water.
Recommended dilutions for use
1: 3000 with standard ECL (WB)
Molecular weight (expected | аpparent)
30.7 | 30 kDa (Zea mays)
Verified applications
western blot (WB)
TAIR number
Refer to NCBI
Clone
Polyclonal
Clonality
Polyclonal
Available ordering format
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Raised in
Rabbit
Protein number
Q9ATM4
Purification
Serum