Description

Size

100 µl

Catalog no.:

AS12 2110

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All details

Possible reactivity

Arabidopsis thaliana, Brassica oleracea, Cicer arietinum, Coffea arabica, Cucumis sativus, Fragaria chiloensis, Glycine max, Medicago trunculata, Mimosa pudica, Nicotiana tabacum, Olea europaea, Phaseolus vulgaris, Pisum sativum, Pyrus communis, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, Triticum urartu, Vitis vinifera,monocots: Hordeum vulgare, Oryza sativa, Triticum aestivum, trees: Picea mariana, Populus trichocarpa

Immunogen

KLH-conjugated synthetic peptide derived from Zea mays PIP2-7 C-terminal, Q9ATM4, conserved also in Zea mays PIP2-1, UniProt: Q84RL7, PIP2-2, UniProt: Q9ATM8, PIP2-3 (80 % conservation) UniProt: Q9ATM7, PIP2-4 (80 % conservation) UniProt: Q9ATM6, PIP2-5 (70 % conservation) UniProt: Q9XF58, PIP2-6 (50 % conservation) UniProt: Q9ATM5

Description

Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae. 

Scientific context

Plasma membrane aquaporin, PIP2;7 is water channel protein required for water transport across cell membrane. Alternative names: plasma membrane intrinsic protein 2-7, AtPIP2;7, plasma membrane intrinsic protein 3, salt stress-induced major intrinsic protein, PIP3a

Storage condition

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Connected products

AS09 488 | PIP2;1 | aquaporin PIP2;1AS09 490 | PIP2;2 | plasma membrane aquaporin 2bAS09 491 | PIP2;1,PIP2;2,PIP2;3 |plasma membrane intrinistic protein 2-1,2-2, 2-3Plant protein extraction bufferSecondary antibodies

Notes

Protocol for isolation of plant plasma membrane proteins can be found here.This antibody has a potential to work in immunolocalization studies, as it is recognizing C-terminal part of the sequence.

No reactivity

no confirmed exceptions from predicted reactivity known in the moment

Verified reactivity

Lactuca sativa, Pisum sativum, Solanum lycopersicum, Zea mays

References

to be added when available, antibody released in May 2012.

Supplementary information

detection pattern consists of di and monomer of PIP2-7

How to reconstitute

For reconstitution add 100 µl of sterile water.

Recommended dilutions for use

1: 3000 with standard ECL (WB)

Molecular weight (expected | аpparent)

30.7 | 30 kDa (Zea mays)

Verified applications

western blot (WB)

TAIR number

Refer to NCBI

Clone

Polyclonal

Clonality

Polyclonal

Available ordering format

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Raised in

Rabbit

Protein number

Q9ATM4

Purification

Serum