Description
Size
96 Tests
Catalog no.:
EKE62261
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All details
Test Principle
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat RBP4 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat RBP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat RBP4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat RBP4. The concentration of Rat RBP4 in samples can be calculated by comparing the OD of the samples to the standard curve.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Shelf life
Use Rat RBP4 (Retinol Binding Protein 4, Plasma) ELISA Kit before 6 months
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Cross Activity
No significant cross-reactivity or interference was observed.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Quality Systems
The kit is manufactured at ISO 9001 certified facilities.
Research Area
Cancer, Cardiovascular, Metabolism, Signal transduction
Application
For research use only. Not for diagnostic procedures.
Storage Temperature
Short term: 4°C; Long term: see manual.
Sample Type
Serum, Plasma, Biological Fluids
Estimated Turnaround Time
6-11 business days
Latin name
Rattus norvegicus
Detection Range
1.56-100 ng/mL
Detection Method
Colorimetric
Sensitivity
0.94 ng/mL
Shipping Conditions
Ice packs
Assay Time
4.5 hours
Assay Type
Sandwich
Target's alterntive name
RBP4
Species Reactivity
Rat
Target Name
NA
Specificity
NA
Stability
NA
Precision
NA