Description

Size

96 Tests

Catalog no.:

EKE62261

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All details

Test Principle

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat RBP4 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat RBP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat RBP4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat RBP4. The concentration of Rat RBP4 in samples can be calculated by comparing the OD of the samples to the standard curve.

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Shelf life

Use Rat RBP4 (Retinol Binding Protein 4, Plasma) ELISA Kit before 6 months

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Cross Activity

No significant cross-reactivity or interference was observed.

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Quality Systems

The kit is manufactured at ISO 9001 certified facilities.

Research Area

Cancer, Cardiovascular, Metabolism, Signal transduction

Application

For research use only. Not for diagnostic procedures.

Storage Temperature

Short term: 4°C; Long term: see manual.

Sample Type

Serum, Plasma, Biological Fluids

Estimated Turnaround Time

6-11 business days

Latin name

Rattus norvegicus

Detection Range

1.56-100 ng/mL

Detection Method

Colorimetric

Sensitivity

0.94 ng/mL

Shipping Conditions

Ice packs

Assay Time

4.5 hours

Assay Type

Sandwich

Target's alterntive name

RBP4

Species Reactivity

Rat

Target Name

NA

Specificity

NA

Stability

NA

Precision

NA