Description
Size
96T
Catalog no.:
ELK5021
Just click and buy
Quick buy process
We invite you to purchase this product. Our professional team will fulfill your order as soon as possible. Our experienced employees will take care of your order very quickly.
All details
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Selenoprotein P1, Plasma (SEPP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Selenoprotein P1, Plasma (SEPP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Selenoprotein P1, Plasma (SEPP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Selenoprotein P1, Plasma (SEPP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Research Area
Metabolic pathway;Infection immunity;
Alternative Names
SEPP; SeP; SEP-P1; SELP
Detection range
0.78-50ng/mL
Sensitivity
0.37ng/mL
Assay Type
Sandwich
Standard
50ng/mL
Assay length
3h