Description

Size

96T

Catalog no.:

ELK6045

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All details

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinol Binding Protein 4, Plasma (RBP4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Retinol Binding Protein 4, Plasma (RBP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Retinol Binding Protein 4, Plasma (RBP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Retinol Binding Protein 4, Plasma (RBP4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Research Area

Metabolic pathway;Endocrinology;Hormone metabolism;

Alternative Names

PRBP; RBP; Plasma retinol-binding protein

Detection range

0.312-20ng/mL

Sensitivity

0.141ng/mL

Assay Type

Sandwich

Standard

20ng/mL

Assay length

3h