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ELISA: Antibody detection limit dilution 1:64000 Western Blot: Approximately 48 kDa and 28 kDa in HepG2 lysates after 1 ug/mL antibody staining.8 kDa according to NP_000069.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
CETP antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
CETP antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
CETP antibody was made against a 14 amino acid synthetic peptide near the N-terminus of CETP.
CETP, lipid transfer protein, HDLCQ10, cholesteryl ester transfer protein, plasma, BPIFF
Aliquot and store at -20 grades C. Minimize freezing and thawing.
cholesteryl ester transfer protein, plasma
ELISA, Western Blot