Description
Size
200 µl
Catalog no.:
AS06 120
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Scientific context
A copper-containing protein in Chlamydomonas reinhardtii is a plasma membrane ferroxidase, encoded by the FOX1 gene, involved in Fe assimilation. FOX1 is most highly related to the mammalian homologues hephaestin and ceruloplasmin; its occurrence and pattern of expression in Chlamydomonas indicate a role for copper in iron assimilation in a photosynthetic species.
References
Kropat et al. (2015). Copper economy in Chlamydomonas: Prioritized allocation and reallocation of copper to respiration vs. photosynthesis. Proc Natl Acad Sci U S A. 2015 Feb 2. pii: 201422492.LaFontaine et al. (2002) Copper-Dependent Iron Assimilation Pathway in the Model Photosynthetic Eukaryote Chlamydomonas reinhardtii. Eukaryotic Cell 1(5):736-757
Supplementary information
This antibody has been used on Chlamydomonas cells expressing endogenous Fox1 and on HEK293 cells transiently expressing Fox1. Cells were resuspended in PBS plated on poly-lysine coated coverslips, fixed in 3.7% formaldehyde. Blocked in 3% BSA in PBS. 1:500 dilution of anti-FOX1 in 3% BSA. Secondary antibody was anti-rabbit Alexa fluor 488 1:300.
Description
Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae.
Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Notes
FOX1 is 10-20-fold induced in iron-deficiency and its occurrence therefore serves as a marker of iron nutrition state in Chlamydomonas. For detection image please refer to the article below.
Connected products
AS07 260 | anti-H+ATPase, plasma membrane rabbit marker antibodyCollection of antibodies to Chlamydomonas proteinsAlgal protein extraction buffer Secondary antibodies
Immunogen
residues 394-646 from Chlamydomonas reinhardtii FOX1 A8IZT9 fused to TrxA
No reactivity
no confirmed exceptions from predicted reactivity known in the moment
How to reconstitute
For reconstitution add 200 µl of sterile water.
Verified applications
western blot (WB), immunofluorescence (IF)
Recommended dilutions for use
1: 1000 - 1: 5000 with standard ECL (WB),
Verified reactivity
Chlamydomonas reinhardtii
Possible reactivity
Chlamydomonas reinhardtii
Protein number
394646 , A8IZT9
TAIR number
Refer to NCBI
Molecular weight (expected | аpparent)
122 | 120 kDa
Available ordering format
Lyophilized
Clonality
Polyclonal
Clone
Polyclonal
Raised in
Rabbit
Purification
Serum