Description

Size

100 µl

Catalog no.:

AS09 491

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Notes

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Protocol for plasma membrane isolation can be found here.

Scientific context

PIP2;2 is a plasma membrane aquaporin. Alternative names of isoforms: aquaporin PIP2-1, plasma membrane intrinsic protein 2a, PIP2a, aquaporin PIP2-2, plasma membrane intrinsic protein 2b, PIP2b, TMP2b, Aquaporin PIP2-3, plasma membrane intrinsic protein 2c, PIP2c, TMP2C, RD28-PIP, water stress-induced tonoplast intrinsic protein, (WSII-TIP)

Description

Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae. 

Supplementary information

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Manufactured by Operon Biotechnologies.

Possible reactivity

Camelina sativa, Brassica napus, Cucumis sativus, Glycine hispida, Gossypium hirsutum, Hedychium coronarium, Mimosa saman, Nicotiana glauca, Petunia hybrida, Pisum sativum, Ricinus communis, trees: Populus tremula x Populus tremloides, moss: Physcomitrella patens

References

Chowanski et al. (2015). Cold induced changes in lipid, protein and carbohydrate levels in the tropical insect Gromphadorhina coquereliana. Comp Biochem Physiol A Mol Integr Physiol. 2015 May;183:57-63. doi: 10.1016/j.cbpa.2015.01.007. Epub 2015 Jan 23.

Connected products

collection of antibodies to plasma membrane proteins AS09 488 | anti-PIP2;1 | aquaporin PIP2;1AS09 489 | anti-PIP1;1, PIP1;2, PIP1;3 | aquaporinsAS09 490 | anti-PIP2;2 | plasma membrane aquaporin 2bPlant protein extraction bufferSecondary antibodies

Storage condition

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PIP2 proteins: AtPIP2-1, At3g53420 , AtPIP2-2 P43287, At2g37170 , AtPIP2-3 P30302, At2g37180

No reactivity

no confirmed exceptions from predicted reactivity known in the moment

Verified reactivity

Arabidopsis thaliana, Gromphadorhina coquereliana, Raphanus sativus

How to reconstitute

See included datasheet or contact our support service

Recommended dilutions for use

1: 8000 (ELISA), 1: 1000 with standard ECL (WB)

Molecular weight (expected | аpparent)

30.4 | 28 (PIP2-1,PIP2-2,PIP2-3) kDa

Verified applications

ELISA (ELISA), western blot (WB)

Protein number

P43286 , P43287 , P30302

Clone

Polyclonal

Clonality

Polyclonal

TAIR number

AT3G53420

Available ordering format

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Raised in

Rabbit

Purification

Serum