Description
Size
100 µl
Catalog no.:
AS09 505
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Connected products
collection of antibodies to plasma membrane proteins AS09 504 | anti-PIP1;3 | aquaporin, plasma membrane intrinistic protein 1-3AS09 506 | anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1AS09 507 | anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1AS09 508 | anti-PIP2;5 | aquaporin, plasma membrane intrinistic protein 2-5Secondary antibodies
Notes
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Protocol for isolation of plasma membrane is available here.
References
Liu et al. (2013). Brittle Culm1, a COBRA-Like Protein, Functions in Cellulose Assembly through Binding Cellulose Microfibrils. PLoS Genet 9(8): e1003704. doi:10.1371/journal.pgen.1003704 (Oryza sativa, western blot)Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39.
Description
Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae.
Supplementary information
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Manufactured by Operon Biotechnologies.
Storage condition
store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Scientific context
PIP1s - is a plasma membrane aquaporine which facilitates trasport of water across cell membrane.
No reactivity
no confirmed exceptions from predicted reactivity known in the moment
Immunogen
KLH-conjugated peptide derived from Oryza sativa PIP1s
How to reconstitute
See included datasheet or contact our support service
Recommended dilutions for use
1: 8000 (ELISA), 1: 2000 with standard ECL (WB)
Verified applications
ELISA (ELISA), western blot (WB)
Molecular weight (expected | аpparent)
26-29 kDa (Oryza sativa)
Possible reactivity
not determined
Protein number
Refer to NCBI
TAIR number
Refer to NCBI
Verified reactivity
Oryza sativa
Clone
Polyclonal
Clonality
Polyclonal
Available ordering format
Inquire
Raised in
Rabbit
Purification
Serum