Description

Size

100 µl

Catalog no.:

AS09 506

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Notes

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.For immunogold technique, secondary antibodies conjugated with coloidal gold, 10 nm particle size were used.Protocol for isolation of plasma membrane can be found here.

Supplementary information

Antibody works very well on samples from roots. Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Manufactured by Operon Biotechnologies.

Description

Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae. 

Connected products

collection of antibodies to plasma membrane proteins AS09 505 | anti-PIP1s | aquaporinAS09 507 | anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1AS09 508 | anti-PIP2;5 | aquaporin, plasma membrane intrinistic protein 2-5Plant protein extraction bufferSecondary antibodies

Storage condition

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Scientific context

PIP2;3 is an aquaporin which facilitate the transport of water and small neutral molecules across cell membrane. Alternative names: plasma membrane intrinsic protein 2-3, OsPIP2;3

References

Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39.

Immunogen

KLH-conjugated peptide derived from Oryza sativa PIP2;3 Q7XUA6

Recommended dilutions for use

1: 8000 (ELISA), 1: 2000 with standard ECL (WB), 1: 100 (IG)

How to reconstitute

See included datasheet or contact our support service

Verified applications

ELISA (ELISA), western blot (WB), immunogold (IG)

Verified reactivity

Oryza sativa, Zea mays

No reactivity

Arabidopsis thaliana

Molecular weight (expected | аpparent)

31.9 | 26-29 kDa

TAIR number

Refer to NCBI

Clone

Polyclonal

Clonality

Polyclonal

Possible reactivity

monocots

Available ordering format

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Raised in

Rabbit

Protein number

Q6Z2T3

Purification

Serum