Description
Size
100 µl
Catalog no.:
AS09 506
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Notes
0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.For immunogold technique, secondary antibodies conjugated with coloidal gold, 10 nm particle size were used.Protocol for isolation of plasma membrane can be found here.
Supplementary information
Antibody works very well on samples from roots. Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Manufactured by Operon Biotechnologies.
Description
Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae.
Connected products
collection of antibodies to plasma membrane proteins AS09 505 | anti-PIP1s | aquaporinAS09 507 | anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1AS09 508 | anti-PIP2;5 | aquaporin, plasma membrane intrinistic protein 2-5Plant protein extraction bufferSecondary antibodies
Storage condition
store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Scientific context
PIP2;3 is an aquaporin which facilitate the transport of water and small neutral molecules across cell membrane. Alternative names: plasma membrane intrinsic protein 2-3, OsPIP2;3
References
Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39.
Immunogen
KLH-conjugated peptide derived from Oryza sativa PIP2;3 Q7XUA6
Recommended dilutions for use
1: 8000 (ELISA), 1: 2000 with standard ECL (WB), 1: 100 (IG)
How to reconstitute
See included datasheet or contact our support service
Verified applications
ELISA (ELISA), western blot (WB), immunogold (IG)
Verified reactivity
Oryza sativa, Zea mays
No reactivity
Arabidopsis thaliana
Molecular weight (expected | аpparent)
31.9 | 26-29 kDa
TAIR number
Refer to NCBI
Clone
Polyclonal
Clonality
Polyclonal
Possible reactivity
monocots
Available ordering format
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Raised in
Rabbit
Protein number
Q6Z2T3
Purification
Serum