Cleaverplas

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Protocol for isolation of plasma membrane can be found here

collection of antibodies to plasma membrane proteins AS09 504 | anti-PIP1;3 | aquaporin, plasma membrane intrinistic protein 1-3AS09 505 | anti-PIP1s | aquaporinAS09 506 | anti-PIP2;1 | aquaporin, plasma membrane intrinistic protein 2-1AS09 508 | anti-PIP2;5 | aquaporin, plasma membrane intrinistic protein 2-5Plant protein extraction bufferSecondary antibodies

Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae. 

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Manufactured by Operon Biotechnologies.

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39.

PIP2;1 is an aquaporin which facilitate the transport of water and small neutral molecules across cell membrane.

no confirmed exceptions from predicted reactivity known in the moment

See included datasheet or contact our support service

KLH-conugated ptide derived from Oryza sativa PIP2-1

1: 8000 (ELISA), 1: 2000 with standard ECL (WB)

ELISA (ELISA), western blot (WB)

30.2 | 26-29 kDa

Refer to NCBI

Oryza sativa

Oryza sativa

Polyclonal

Polyclonal

Inquire

Rabbit

Q7XUA6

Serum