Description

Size

1000ug

Catalog no.:

GEN1178678.Yeast

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All details

General description

Biotin synthase (bioB) is a recombinant protein expressed in Yeast . The protein can be with or without a His-Tag or other tag in accordance to customer's request. All of our recombinant proteins are manufactured in strictly controlled facilities and by using a well established technology which guarantees full batch-to-bact consistency and experiment reproducibility.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Storage

This protein can be stored at -20 degrees Celsius. For extended periods of time it is recommended to keep the protein frozen at -40 or -80 degrees Celsius. Avoid cycles of freezing and thawing as they might denaturate the polypeptide chains.

Applications

This protein can be used as a positive control for applications such as ELISA, IFA, RIA, Western Blot, etc.

Long name

Recombinant Anaplasma marginale Biotin synthase (bioB)

Alternative names

biotin synthase; Biotin synthase; biotin synthase;

Available also expressed in:

E Coli ; Yeast ; Baculovirus ; Mammalian Cell

Purity

Greater than 90% (determined by SDS-PAGE)

Product category

Recombinant Proteins

Form

Lyophilized protein

Conjugation

Biotinylated

Other gene names

bioB; bioB;

Expression system

Yeast

Gene name

bioB