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Mycoplasma is a genus of bacteria that lack a cell wall around their cell membrane. Without a cell wall, they are unaffected by many common antibiotics such as penicillin or other beta-lactam antibiotics that target cell wall synthesis. They can be parasitic or saprotrophic. Several species are pathogenic in humans, including M. pneumoniae, which is an important cause of atypical pneumonia and other respiratory disorders, and M. genitalium, which is believed to be involved in pelvic inflammatory diseases. Mycoplasma species are the smallest bacterial cells yet discovered, can survive without oxygen, and come in various shapes. For example, M. genitalium is flask-shaped (about 300 x 600 nm), while M. pneumoniae is more elongated (about 100 x 1000 nm). Hundreds of mycoplasmas infect animals
This molecular diagnostic PCR kit is CE approved and complies with the Directive 98/79/EC for IVD, in vitro PCR Diagnostics. The PCR kit is ISO 9001:2008 and ISO 13485:2012, CE marked with the in vitro diagnostic medical divices requirements Annex 2 of list B of the laboratory Directive 98/79/EC and can be used for diagnosis of viruses. The kit inculde all reagents for PCR or RT-PCR of viral DNA or RNA.
This Mycoplasma genitalium 280/550 IC PCR kit with Electrophoretic Detection for detection of M.genitalium is used as PCR kit for molecular diagnostics with elecrtophoresis, qPCR or real-time PCR of 110 Tests per screening.
The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
PCR, polymerase chain reaction